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时间：2023年3月14日（周二） 上午10:30-12:00

Time: 10:30am-12:00pm，Tuesday, March 14th, 2023

地点：E10-211, 云谷校区

Venue：E10-211, Yungu Campus

主持人: 华体会官方网站,华体会官方网站登录入口,华体会网页版登录入口,华体会手机版登录 工学院 讲席教授Mohamad Sawan

Host: Mohamad Sawan, Chair Professor of School of Engineering, Westlake University

语言：英文

Language: English

主讲嘉宾/Speaker：

Dr. Jingyun Li

Principal Investigator

SRRS Hospital

主讲人简介/Biography:

Jingyun obtained her PhD degree at Peking university and did postdoctoral research at Boston Children's Hospital and Harvard Medical School for three years. She joined the Sir Run Run Shaw Hospital (浙江大学医学院附属邵逸夫医院) as a principal investigator since Feb 2022. Jingyun finished her PhD training at Prof Fuchou Tang's lab, during which she developed a single cell multi-omic sequencing methods (Cell Research, 2017), and dissected the regulatory programs at multiple stages during carcinogenesis of colorectal cancer (Gut, 2020; Genome Medicine, 2022; Nature Cell Biology, 2018;). Then, she gained expertise in organoids systems at Carla Kim Lab (Cancer cell, under review). Her future research will focus on using the organoids system to investigate the mechanisms underlying cancer cell plasticity, and aim to find targets for cell plasticity during drug resistance and relapse.

讲座摘要/Abstract:

Single cell profiling assays have greatly enhanced the dissection of tumor heterogeneity. Multiple cell states have been found to exist in primary lung adenocarcinoma. However, the functional differences among these various cell states have not been well studied. It has been challenging to answer this question using traditional cancer cell lines or mouse models, since cell lines can hardly maintain the heterogeneity or chromatin features of primary tumors, while working with mouse models is time-consuming. We established a lung tumor organoids system, which has been verified to recapitulate the early transcriptome hallmarks of the in vivo counterparts (Dost et al., Cell Stem Cell. 2020). Here, I will discuss our recent work on investigating the potential of using this organoids system to model tumor heterogeneity. We found that our tumor organoids system can recapitulate the tumor heterogeneity of the in vivo counterparts at both gene expression level and chromatin accessibility level. Then, we combined gene expression assay and chromatin accessibility assay to reveal potential molecular mechanisms underlying tumor heterogeneity, and systematically evaluated the tumorigenic capacity of different tumor cell states. Furthermore, we found that the cell states we identified in murine tumor organoids have similar analogues in early-stage human LUAD, which demonstrates the utility of using our organoids system as an approach to identify and probe the critical regulators of cell state and plasticity that may be driving human lung cancer.

讲座联系人/Contact:

Yitian(Claire) Zhang

zhangyitian@jsyjsq.cn